Trying to tweak a large drive to get more storage space is a really bad idea! Unless you're a certified Software Engineer, accept the default, 4096. Saving huge files, like videos, for example, DEMANDS NTFS.
Older settings, in their day were useful, because they were the maximum allowed at the time. But today's huge drives always work best left untouched.
But, do NOT use any 3rd party tools, like Acronis (unnecessary) other than Windows' built-in Partitioning tool, OR a free Utility provided by the hard drive manufacturer. Fdisk, an old DOS utility, can actually damage newer HDDs, for example.
TIP: XHDs are formatted to FAT-32 at the factory for good reason, it's the only one that is usable on Windows, OS X AND Linux, and works best for backing up all your stuff.